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H-start for exclusively heteronuclear NMR spectroscopy: The case of intrinsically disordered proteins |
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| Authors: | Wolfgang Bermel Ivano Bertini Veronika Csizmok Isabella C. Felli Roberta Pierattelli Peter Tompa |
| Affiliation: | aBruker BioSpin GmbH, Silberstreifen, 76287 Rheinstetten, Germany;bCERM and Department of Chemistry, University of Florence, Via Luigi Sacconi 6, IT-50019 Sesto Fiorentino, Florence, Italy;cInstitute of Enzymology, Biological Research Center, Hungarian Academy of Sciences, Budapest, Karolina ut 29, H-1113, Hungary |
| Abstract: | Here, we present a series of exclusively heteronuclear multidimensional NMR experiments, based on 13C direct detection, which exploit the 1H polarization as a starting source to increase the signal-to-noise ratio. This contributes to make this spectroscopy more useful and usable. Examples are reported for a suitable system such as securin, an intrinsically disordered protein of 22 kDa. |
| Keywords: | 13C direct detection Protonless NMR Intrinsically disordered proteins Virtual decoupling IPAP Spin-state selection |
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