High-performance liquid chromatography with post-column derivatisation and fluorescence detection for sensitive determination of aflatoxin M1 in milk and cheese |
| |
Authors: | Manetta Anna Chiara Di Giuseppe Lorella Giammarco Melania Fusaro Isa Simonella Anselmo Gramenzi Alessandro Formigoni Andrea |
| |
Institution: | Department of Food and Feed Science, University of Teramo, Viale F. Crispi 212, 64100 Teramo, Italy. a_manetta@virgilio.it |
| |
Abstract: | A new HPLC method with fluorescence detection using pyridinium hydrobromide perbromide as a post-column derivatising agent has been developed to determine aflatoxin M1 in milk and cheese. The detection limits were 1 ng/kg for milk and 5 ng/kg for cheese. The calibration curve was linear from 0.001 to 0.1 ng injected. The method includes a preliminary C18-SPE clean-up and the average recoveries of Aflatoxin M1 from milk and cheese, spiked at levels of 25-75 ng/kg and 100-300 ng/kg, respectively, were 90 and 76%; the precision (RSDr) ranged from 1.7 to 2.6% for milk and from 3.5 to 6.5% for cheese. The method is rapid, easily automatable and therefore useful for accurate and precise screening of aflatoxin M1 in milk and cheese. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|