Total internal reflection ellipsometry as a label-free assessment method for optimization of the reactive surface of bioassay devices based on a functionalized cycloolefin polymer |
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Authors: | Nam Cao Hoai Le Vladimir Gubala Ram P Gandhiraman Conor Coyle Stephen Daniels David E Williams |
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Institution: | (1) Biomedical Diagnostics Institute (BDI), Dublin City University, Dublin 9, Republic of Ireland;(2) National Centre for Plasma Science and Technology (NCPST), Dublin City University, Dublin 9, Republic of Ireland;(3) MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Chemistry, University of Auckland, Auckland, 1142, New Zealand |
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Abstract: | We report a label-free optical detection technique, called total internal reflection ellipsometry (TIRE), which can be applied
to study the interactions between biomolecules and a functionalized polymer surface. Zeonor (ZR), a cycloolefin polymer with
low autofluorescence, high optical transmittance and excellent chemical resistance, is a highly suitable material for optical
biosensor platforms owing to the ease of fabrication. It can also be modified with a range of reactive chemical groups for
surface functionalization. We demonstrate the applications of TIRE in monitoring DNA hybridization assays and human chorionic
gonadotrophin sandwich immunoassays on the ZR surface functionalized with carboxyl groups. The Ψ and Δ spectra obtained after the binding of each layer of analyte have been fitted to a four-layer ellipsometric model to quantitatively
determine the amount of analytes bound specifically to the functionalized ZR surface. Our proposed TIRE technique with its
very low analyte consumption and its microfluidic array format could be a useful tool for evaluating several crucial parameters
in immunoassays, DNA interactions, adsorption of biomolecules to solid surfaces, or assessment of the reactivity of a functionalized
polymer surface towards a specific analyte. |
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