Amperometric biosensors for glucose based on carbon paste modified electrodes |
| |
Authors: | Amine A Kauffmann J M Patriarche G J |
| |
Institution: | Université Libre de Bruxelles, Institut de Pharmacie, Campus Plaine, CP 205/6, Bd. du Triomphe, 1050 Bruxelles, Belgium. |
| |
Abstract: | The modification of carbon-paste electrodes by incorporation of the enzyme glucose oxidase (GOD) is described. The resulting probes can be operated as amperometric glucose sensors in the presence or absence of a mediator (1,1'-dimethylferrocene) mixed into the paste. Extended linear calibration ranges have been obtained up to 90 and 5OmM glucose respectively. The electrode responses were rapid, reaching steady-state values within 30-40 sec. Advantages of using a GOD-paste formulation are suggested. Plasma glucose assays were correlated with spectrophotometric determinations based on glucose oxidase (y = 1.07x - 0.16, r = 0.973, n = 17). |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|