Electrochemical Detection of MS2 Phage Using a Bead‐based Immunoassay and a NanoIDA |
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Authors: | Adam Bange Jian Tu Xiaoshan Zhu Chong Ahn H?Brian Halsall William?R Heineman |
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Institution: | 1. Department of Chemistry, University of Cincinnati, P.?O. Box 210172, Cincinnati, Ohio, 45221‐0172, USA;2. Department of Electrical Engineering, University of Cincinnati, Cincinnati, Ohio, 45221, USA |
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Abstract: | A microbead based sandwich immunoassay for MS2 bacteriophage was developed using an interdigitated array (IDA) electrode with nanoscale dimensions (220 nm electrode width, 620 nm gap). The IDA was fabricated using an electron beam lithographic lift‐off technique. After an antibody‐assisted capture of MS2 using paramagnetic microbeads, a β‐galactosidase labeled secondary antibody was used to convert p‐aminophenyl‐β‐D ‐galactopyranoside (PAPG) into the redox active p‐aminophenol (PAP). Amperometric detection of PAP with IDA electrodes at +300 and ?200 mV vs. a Ag/AgCl reference electrode was used to measure the result, detecting MS2 concentrations as low as 10 ng/mL. |
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Keywords: | Interdigitated array electrode Immunoassay Nanoscale |
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