Polyphenothiazine Modified Electrochemical Aptasensor for Detection of Human α‐Thrombin |
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| Authors: | Anna Porfirieva Gennady Evtugyn Tibor Hianik |
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| Affiliation: | 1. Department of Nuclear Physics and Biophysics, Comenius University, Mlynska dolina F1, 842 48 Bratislava, Slovakia;2. Department of Chemistry, Kazan State University, Kremlevskaya Str. 18. 420008?Kazan, Russia |
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| Abstract: | ![]()
QCM aptasensor for detection human thrombin has been developed on the base of polymeric forms of phenothiazine dyes, Methylene Blue and Methylene Green. Electrostatic accumulation of the analyte in the polyphenothiazine layer made it possible to increase the sensitivity of QCM detection of thrombin in comparison with bare gold electrodes coated with avidin or neutravidin. The influence of nonspecific binding of human serum albumin and the optimal composition of the surface layers were determined. The aptasensors developed make it possible to detect 10–100 nM of thrombin. |
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| Keywords: | Thrombin DNA Aptamer Methylene Blue Methylene Green QCM |
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