Amperometric Glucose Determination by Means of Glucose Oxidase Immobilized on a Cellulose Acetate Film: Dependence on the Immobilization Procedures |
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| Authors: | Marianna Portaccio Daniela Durante Andrea Viggiano Silvana Di?Martino Paola De?Luca Daniela Di?Tuoro Umberto Bencivenga Sergio Rossi Paolo Canciglia Bruno De?Luca Damiano?Gustavo Mita |
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| Institution: | 1. Department of Experimental Medicine, Faculty of Medicine and Surgery, Second University of Naples, Via S.?M. di Costantinopoli, 16‐80136?Naples, Italy;2. National Institute of Biostructures and Biosystems “INBB” – Viale Medaglie d'oro, 305‐00136 Rome, Italy;3. Institute of Genetics and Biophysics of CNR, Via Pietro Castellino,111‐80131?Naples, Italy;4. Department of Pharmacobiology, University of Messina, Via S.?S. Annunziata 90168?Messina, Italy |
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| Abstract: | Glucose microelectrodes were prepared by immobilizing glucose oxidase onto a cellulose acetate film coating a platinum wire. Hexamethylenediamine (HMDA) and Glutaraldehyde (GA) were employed as spacer and coupling agent, respectively. Sensitivities and linear response ranges were studied as a function of the relative amounts of HMDA and GA. The best sensitivity was found when HMDA and GA were 5% and 2.5% in aqueous solutions, respectively. Taking as a reference the functioning of this biosensor, the roles of HMDA and GA percentages appear to be opposed when the extension of the linear response range is considered. Indeed, an increase of one unit in HMDA percentage (from 5 to 6 %) induces an increase in the extension of the linear response range equal to that obtained with a decrease of one unit of GA percentage (from 2.5 to 1.5%). |
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| Keywords: | Cellulose acetate Enzyme immobilization Glucose biosensor Glucose oxidase Glucose determination |
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