A Calibration‐Base Method for the Evaluation of the Detection Limit of an Electrochemical Biosensor

The method based on the confidence limits of a regression straight line is applied to the linear portion of the calibration curve of a biosensor in order to determine the limit of detection. A practical application gives a detection limit of an ethanol biosensor greater than that calculated by using the method of the signal‐to‐noise‐ratio, S/N=3, but the value is more reliable because it accounts for the variability in the response at analyte additions. A simple approximation of the method is also presented.