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Cobalt Phthalocyanine Molecular Electrode for the Electrochemical Investigation of the Release of Glutathione upon Copper‐Catalyzed Decomposition of S‐Nitrosoglutathione |
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| Authors: | Nthapo Sehlotho Sophie Griveau Tebello Nyokong Fethi Bedioui |
| Affiliation: | 1. INSERM, U640, CNRS, UMR8151, René Descartes Paris 5, Chemical and Genetic Pharmacology Laboratory, école Nationale Supérieure de Chimie de Paris, Paris, France;2. Rhodes University, Department of Chemistry, Grahamstown, South Africa |
| Abstract: | Decomposition of S‐nitrosoglutathione (GSNO) in phosphate buffer solution at physiological pH 7.4 in the presence of cuprous ion as a catalyst and sodium borohydride as a reducing agent is analyzed by observing the transient apparition of reduced glutathione GSH through its electrooxidation. Transient formation of GSH, upon decomposition of 1 mM GSNO in presence of 0.025 mM Cu(NO3)2 and 1 mM NaBH4 was detected by using an ordinary pyrolytic graphite electrode modified with an adsorbed monolayer of cobalt phthalocyanine at 0 V vs. SCE. |
| Keywords: | Cobalt phthalocyanine S‐Nitrosoglutathione Reduced glutathione |