烷基吡啶盐和芳基季铵盐作检测和改性剂的羟基酸间接光度高效液相色谱研究

研究了苄基三甲基溴化铵动态改性、十四烷基吡啶盐等“永久性”涂渍的两种反相色谱,系统分离和间接光度检测了强极性、弱紫外吸收羟基酸。测定这些检测和改性试剂在YWG-CH反相填料和甲醇-磷酸缓冲液色谱体系的吸附等温线,探讨试剂在固定相的吸附量和其它因素对羟基酸保留、检测灵敏度的影响及分离检测机理。

Indirect Photometric Chromatography of Hydroxy Acids Using UV-AbsorbingAlkylpyridinium or Quaternary Ammonium Salts as DetectionAgents and Modifiers

The reversed- phase chromatographic systems, either modified dynamically with ben-zyltrimethyl ammonium bromide or coated "permanently" with alkylpyridinium salts, have been studied for the separation and detection of hydroxy acids by the indirect photometric detecction. The ben- zyltrimethyl ammonium bromide and alkylpyridinium salts would play the part of a detection agent and a modifier in the indirect photometric chromatography. The adsorption isotherms of these reagents on YWG-CH reversed phase packing in methanol-aqueous phosphate buffer solutions are determined. The influence of the amounts of the reagents adsorbed on the stationary phase and that of other factors on the retention and detection sensitivity of hydroxy acids are illustrated.