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Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer-peptide conjugate |
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| Authors: | Ohtsuka Keiichi Sato Shinobu Sato Yusuke Sota Kojiro Ohzawa Shinsuke Matsuda Tomoki Takemoto Kiwamu Takamune Nobutoki Juskowiak Bernard Nagai Takeharu Takenaka Shigeori |
| Affiliation: | Department of Applied Chemistry, Graduate School of Engineering, Kyushu Institute of Technology, Fukuoka 804-8550, Japan. |
| Abstract: | When a biotinylated FRET probe based on a peptide-thrombin binding aptamer conjugate was introduced together with streptavidin and biotinylated nuclear export signal peptide into HeLa cells, the resulting ternary complex enabled visualization of K(+) concentration changes in the cell. |
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