The analysis of pharmaceutical bases on a silica stationary phase by capillary electrochromatography using aqueous mobile phases |
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Authors: | N C Gillott M R Euerby C M Johnson D A Barrett P N Shaw |
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Institution: | (1) Bioanalysis and Separation Sciences Group, School of Pharmaceutical Sciences, University Of Nottingham, NG7 2RD Nottingham, UK;(2) AstraZeneca R&D Charnwood, Pharmaceutical and Analytical R&D, Bakewell Road, LE11 5RH Loughborough, Leicestershire, UK;(3) Process Analysis R&D, Bakewell Road, LE11 5RH Loughborough, Leicestershire, UK |
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Abstract: | Summary The capillary electrochromatographic (CEC) separation of a range of pharmaceutical bases was investigated on a commercially
available silica stationary phase using aqueous mobile phases. The effects of mobile phase composition, buffer pH, applied
voltage, and buffer anion on the retention behaviour of these bases were studied. Promising chromatography was obtained at
pH 7.8 but was later found to be irreproducible. However, successful and reproducible chromatography of the bases was achieved
at pH 2.3.
We have previously demonstrated that the addition of mobile phase additives such as TEA-phosphate at low pH values has resulted
in excellent CEC analysis of bases on reversed-phase packing materials. The same approach was applied to the analysis of bases
on the silica phase in order to improve peak shape. Excellent chromatography was obtained for the analysis of strong pharmaceutical
bases such as benzylamine, nortriptyline and diphenhydramine.
The experimental investigations have shown that the CEC separation of a range of pharmaceutical bases can routinely be achieved
with excellent peak shapes and peak efficiencies as high as 320,000 plates m−1. |
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Keywords: | Capillary electrochromatography Pharmaceutical bases Silica stationary phases Aqueous mobile phases |
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