用活性污泥生物合成聚羟基烷酸酯的结构表征

聚β-羟基烷酸酯 ( PHA)是一类具有生物相容性、光学活性、热塑性和完全生物降解性的生物高分子 ,有巨大的应用前景 1] .PHA的结构通式为 O CRH CH2 CO ,R为不同链长的饱和或不饱和的烷基 ,可通过微生物发酵进行合成 2 ,3] .利用污水中的有机物和活性污泥中可积累 PHA的混合微生物群生物合成 PHA,能大大降低 PHA的成本 ,变废为宝 ,是近几年 PHA研究的新热点 4 ,5] .用不同菌种和不同碳源合成的 PHA的结构有较大的差异 ,因污水成分复杂 ,所得 PHA是多种饱和与非饱和羟基烷酸酯的混杂共聚物 .本工作以某纺织厂活性污泥为混…

Structure Characterization of Polyhydroxyalkanoates Produced from Activated Sludge in Waste Water Treatment Process

It is found that a number of microorganisms in activated sludge in the process of waste water treatment can produce a variety of nomo and co poly 3 hydroxyalkanoates with a wide range of properties, which would be one of the methods to low the cost of PHA. In this paper, the biosynthesis of polyhydroxyalkanoates(PHA) by mixed bacterium growth on waste water was studied. The chemical structure of PHA, isolated from activated sludge in waste water treatment process using sodium acetate as attachment carbon source, was analyzed by 1H NMR spectroscopy and gas chromatography mass spectrometry. The PHA produced from activated sludge is a copolymer composed of 9 different monomer units, 3 hydroxybutyrate, 4 hydroxypentanoate, 3 hydroxydodecanoate, 3 hydroxy tetradecanoate, 3 hydroxy 9 hexadecenoate, 3 hydroxyhexadecanoate, 3 hydroxy 11 octadecenoate, 3 hydroxy 9 octade\|cenoate, 3 hydroxyoctadecanoate. WT5HZ]