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Study of operational variables in the submerged growth ofPleurotus ostreatus mushroom mycelium
Authors:MaNu-Tawiah  Wireko  Martin  Antonio M
Institution:(1) Department of Biochemistry, Memorial University of Newfoundland, A1B 3X9 St. John’s, Newfoundland, Canada
Abstract:Pleurotus ostreatus mushroom mycelium was cultivated in submerged culture in shake-flask experiments with acid extract from peat and yeast extract as nutrient sources. Different concentrations of water-diluted peat extract were tested in an attempt to overcome the effect of growth inhibitors apparently present in nondiluted peat extracts. The best results were obtained with a ratio of one part of peat extract diluted with one part of water. Several operating variables were studied to optimize the growth of mycelial biomass ofP. ostreatus. The best results produced approximately 5 g/L dry biomass with a yield of 60% and an efficiency of 33%. These results were obtained in 8 d at 5% (v/v) inoculum ratio, 28°C, pH of 5.0, and 150 rpm.
Keywords:Pleurotus ostreatus            mushroom mycelium  peat extract  submerged fermentation  fermentation operational variables
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