Preparation of Functionalized Fe3O4@SiO2 Magnetic Nanoparticles for Monoclonal Antibody Purification |
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Authors: | HOU Xuemei ZHAO Changjie TIAN Yanlong DOU Shuliang ZHANG Xiang ZHAO Jiupeng |
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Institution: | 1. School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin 150001, P. R. China;
2. Center for Composite Materials, Harbin Institute of Technology, Harbin 150001, P. R. China |
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Abstract: | Magnetic Fe3O4@SiO2 nanoparticles with superparamagnetic properties were prepared via a reverse mi-croemulsion method at room temperature. The as-prepared samples were characterized by transmission electron mi-croscopy(TEM), X-ray diffractometry(XRD), and vibrating sample magnetometry(VSM). The Fe3O4@SiO2 nanoparticles were modified by (3-aminopropyl)triethoxysilane(APTES) and subsequently activated by glutaraldehyde(Glu). Protein A was successfully immobilized covalently onto the Glu activated Fe3O4@SiO2 nanoparticles. The adsorption capacity of the nanoparticles was determined on an ultraviolet spectrophotometer(UV) and approximately up to 203 mg/g of protein A could be uniformly immobilized onto the modified Fe3O4@SiO2 magnetic beads. The core-shell of the Fe3O4@SiO2 magnetic beads decorated with protein A showed a good binding capacity for the chime-ric anti-EGFR monoclonal antibody(anti-EGFR mAb). The purity of the anti-EGFR mAb was analyzed by virtue of HPLC. The protein A immobilized affinity beads provided a purity of about 95.4%. |
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Keywords: | Magnetic Fe3O4@SiO2 Protein A Nanoparticle Monoclonal antibody |
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