基于共振瑞利散射血清蛋白浓度检测的研究

血清中蛋白质浓度在临床诊断中有重要意义，而目前广泛使用的紫外-可见分光光度计、荧光分光光度计等仪器结构复杂、昂贵、体积庞大、耗电量高等因素，都无法满足现场高精度检测的要求。设计了基于四羧基酞菁锌-蛋白质体系的共振瑞利散射光谱检测系统，系统以405 nm宽禁带半导体激光器为激励光源，以475 nm窄带带通滤光片为单色器，以蓝光增强光敏二极管的低噪声高增益光电放大器为探测器。通过实验可知，该溶液强吸收波长为420 nm附近，在该激励光作用下，其共振波长处会产生共振瑞利散射，散射强度与蛋白质的含量成比例，可以利用四羧基酞菁锌为光谱探针的共振散射法来测定血清蛋白，其线性检出范围为10～50 mg·mL-1，检出限为0.001 mg·mL-1。新开发的血清蛋白质测试装置具有体积小、成本低、功耗小、使用方便等优点。

Research on Detecting Concentration of Serum Protein Based on Resonance Rayleigh Scattering

The resonance Rayleigh scattering spectral detection system was designed based on the 2,9,16,23-tetracarboxylate-phthalocyanine zinc and protein system. In the system, excitation light source is 405 nm wide band gap semiconductor lasers, and monochromator is 475 nm narrow-band band-pass filter, and the detector is low-noise and high-gain photoelectric amplifier based on blue-ray enhanced photodiode. Experiment shows that, the solution’s strong absorption wavelength is near 420 nm. Under the action of incentive light, resonance Rayleigh scattering is generated at the resonant wavelength, and the scattering intensity is proportional to the protein content. The system uses 2,9,16,23-tetracarboxylate as the spectrum probe to determine the concentration of serum proteins by resonance Rayleigh scattering method. Its linear detection range is 10 ~ 50 mg·mL-1, and its detection limit is 0.001 mg·mL-1. The newly developed device for detecting concentration of the serum protein has the advantages of small size, low cost, low power consumption, and being easy to use.