A validated gas chromatographic method for the evaluation of enzymatic enantioselectivity in kinetic resolution applications |
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| Authors: | Boersma Ykelien L Scheltinga Paula C Dröge Melloney J Bos Rein Quax Wim J |
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| Institution: | Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, Groningen University Institute for Drug Exploration, The Netherlands. |
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| Abstract: | An enantioselective gas chromatography (GC) method has been developed and validated for determination of the enantiomers of citronellol in kinetic resolution experiments. S-(-)-beta-Citronellol is a precursor of rose oxide. After solid-phase extraction (SPE) with ethyl acetate, the enantiomers of R-(+)-beta-citronellol and S-(-)-beta-citronellol and their corresponding acetate- and butyrate esters were separated through enantioselective GC respectively. The method was validated and found to be reproducible, specific, accurate, and precise. Analyte recoveries and detection limits were also determined. The applicability of this method was shown in a kinetic resolution experiment using lipase A of Bacillus subtilis. |
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