|
|||||
|
|
Identification of novel sesterterpenes by genome mining of phytopathogenic fungi Phoma and Colletotrichum sp. |
|
| Authors: | Lei Gao Koji Narita Taro Ozaki Naoyoshi Kumakura Pamela Gan Atsushi Minami Chengwei Liu Xiaoguang Lei Ken Shirasu Hideaki Oikawa |
| Affiliation: | 1. Division of Chemistry, Graduate School of Science, Hokkaido Universiy, Sapporo 060-0810, Japan;2. College of Chemistry and Molecular Engineering, Peking University, Haidan District, Beijing 100871, China;3. Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science, Kanagawa 230-0045, Japan |
| Abstract: | Two homologous gene clusters for the biosynthesis of sesterterpenes betaestacins were identified from two phytopathogens, Phoma betae and Colletotrichum orbiculare. Heterologous expression of identified oxidation enzymes with previously-characterized PbTS1 (BtcAPb) resulted in the production of seven novel sesterterpenes. Although both strains possessed homologous enzymes, oxidation state of corresponding products were different from each other, suggesting that structural diversification of sesterterpene skeletons might be achieved by these homologous enzymes with different functions. |
| Keywords: | Sesterterpene synthase Genome mining Natural product biosynthesis Phytopathogen Heterologous expression |
| 本文献已被 ScienceDirect 等数据库收录！ | |