| Study of the interaction of Zn^2＋ with Aβ（10-21） by fluorescamine assay |
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| 作者姓名： | Chao Feng Zhang Li Fan Pin Yang |
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| 作者单位： | Chao Feng Zhang Li Fan Pin Yang~* Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education，Institute of Molecular Science，Shanxi University，Taiyuan 030006，China |
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| 基金项目： | This work was supported by the National Natural Science Foundation of China （No. 30470408）. |
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| 摘 要： | Zinc may play a role as a co-factor in the pathogenesis of Alzheimer's disease(AD)through influencing the conformation and neurotoxicity of amyloidβ-protein(Aβ).Using the fluorescamine assay,we show for the first time that Zn~(2 )induced Aβ(10-21) aggregate in a concentration-dependent manner.These results indicate that Aβ(10-21)can be used as an in vitro model in Zn~(2 )- induced Aβaggregation and that the region 10-21 to be the minimal fragment of zinc-binding domain of full length Aβ(1-42).
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| 关 键 词： | Aβ(10-21) Fluorescamine Zn~(2 ) Aggregation |
| 收稿时间： | 2006-05-12 |
Study of the interaction of Zn2+ with Aβ(10-21) by fluorescamine assay |
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| Chao Feng Zhang Li Fan Pin Yang.Study of the interaction of Zn2+ with Aβ(10-21) by fluorescamine assay[J].Chinese Chemical Letters,2007,18(1):97-98. |
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| Authors: | Chao Feng Zhang Li Fan Pin Yang |
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| Institution: | Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China |
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| Abstract: | Zinc may play a role as a co-factor in the pathogenesis of Alzheimer's disease (AD) through influencing the conformation and neurotoxicity of amyloid β-protein (Aβ). Using the fluorescamine assay, we show for the first time that Zn2+ induced Aβ(10-21) aggregate in a concentration-dependent manner. These results indicate that Aβ(10-21) can be used as an in vitro model in Zn2+-induced Aβ aggregation and that the region 10-21 to be the minimal fragment of zinc-binding domain of full length Aβ(1-42). |
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