Synthesis of oligosaccharides catalyzed by thermostable β-glucosidase fromPyrococcus furiosus |
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| Authors: | M A Boon J van der Oost W M de Vos A E M Janssen K van ’t Riet |
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| Institution: | (1) Present address: Department of Chemical Engineering, Tokyo Institute of Technology, Ookayama, Meguro-ku, 152 Tokyo, Japan;(2) Department of Applied Chemistry and Biotechnology, Niigata Institute of Technology, 1719 Fujihashi, Kashiwazaki, 945-1195 Niigata, Japan |
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| Abstract: | Focusing on CO2 fixation, photoautotrophic cultivation of the red algaPorphyridium cruentum was investigated by means of a batch culture under a 5% CO2-enriched atmosphere. The algal growth kinetics was successfully described with a logistic model, and simulation of a continuous
culture under the optimum growth conditions (30°C, 12 klux and 1.18 g-cells/L) showed that the algal CO2-fixation activity could reach 0.66 g-CO2/(L X d). Under the same growth conditions, eicosapentaenoic acid (20:5 n-3, EPA) and arachidonic acid (20:4 n-6, ARA) yields
were similarly calculated to be 3.6 mg-EPA/(L X d) and 6.5 mg-ARA/(L X d), respectively. |
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| Keywords: | Carbon dioxide Porphyridium cruentum environment eicosapentaenoic acid arachidonic acid red alga fatty acid |
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