Matrix effects in the determination of β‐receptor agonists in animal‐derived foodstuffs by ultra‐performance liquid chromatography tandem mass spectrometry with immunoaffinity solid‐phase extraction |
| |
| Authors: | Guomin Wang Jian Zhao Tao Peng Dongdong Chen Cunxian Xi Xiong Wang Jinzhong Zhang |
| |
| Institution: | 1. Chongqing Entry‐Exit Inspection and Quarantine Bureau, Chongqing Engineering Technology Research Center for Import and Export Food Safety, , Chongqing, China;2. Chongqing Center for Disease Control and Prevention, , Chongqing, China;3. Chinese Academy of Inspection and Quarantine, , Beijing, China;4. Clover Technology Group Inc., , Beijing, China;5. College of Resources and Environment, Southwest University, , Chongqing, China |
| |
| Abstract: | Matrix effects in determination of three β‐receptor agonists including salbutamol (SAL), clenbuterol, and terbutaline in animal‐derived foodstuffs were studied by ultra‐performance LC‐MS/MS with cleanup of immunoaffinity SPE column (IAC). Some animal tissue samples including pig liver, swine muscle, and fish muscle were hydrolyzed by the mixed enzyme solution or HCl solution, and the cleanup efficiencies with SAL IAC, MCX SPE column, and C18‐SCX tandem columns were examined and compared by using spiked experiments. The results showed that the matrix effects in the determination of SAL and terbutaline can be eliminated with SAL IAC cleanup, and the average recoveries of SAL were 77.4～81.5%, 79.0～80.3%, and 85.0～87.2% in pig liver, swine muscle, and fish muscle, respectively. The decision limit (ccα) and detection capability (ccβ) for SAL in pig liver were 0.02 and 0.05 μg/kg, respectively. |
| |
| Keywords: | Animal‐derived foodstuff Immunoaffinity solid‐phase extraction Matrix effect β ‐Receptor agonist Ultra‐performance liquid chromatography tandem mass spectrometry |
|
|
