A strategy for screening and identifying mycotoxins in herbal medicine using ultra‐performance liquid chromatography with tandem quadrupole time‐of‐flight mass spectrometry |
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Authors: | Lian‐xiang Fang Ai‐zhen Xiong Rui Wang Shen Ji Li Yang Zheng‐tao Wang |
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Institution: | 1. The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, , Shanghai, China;2. The State Administration of TCM (SATCM) Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, , Shanghai, China;3. Shanghai R&D Centre for Standardization of Chinese Medicines, , Shanghai, China;4. Department of Traditional Chinese Medicine, Shanghai Institute for Food and Drug Control, , Shanghai, China |
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Abstract: | The objective of this study was to develop an effective strategy for screening and identifying mycotoxins in herbal medicine (HM). Here, Imperatae Rhizoma, a commonly used Chinese herb, was selected as a model HM. A crude drug contaminated with fungi was analyzed by comparing with uncontaminated ones. Ultra‐performance LC coupled to tandem quadrupole TOF‐MS (UPLC–Q‐TOF‐MS) with collision energy function was applied to analyze different samples from Imperatae Rhizoma. Then, MarkerLynxTM software was employed to screen the excess components in analytes, compared with control samples, and those selected markers were likely to be the metabolites of fungi. Furthermore, each of the accurate masses of the markers obtained from MarkerLynxTM was then searched in a mycotoxins/fungal metabolites database established in advance. The molecular formulas with relative mass error between the measured and theoretical mass within 5 ppm were chosen and then applied to MassFragmentTM analysis for further confirmation of their structures. With the use of this approach, five mycotoxins that have never been reported in HM were identified in contaminated Imperatae Rhizoma. The results demonstrate the potential of UPLC–Q‐TOF‐MS coupled with the MarkerLynxTM software and MassFragmentTM tool as an efficient and convenient method to screen and identify mycotoxins in herbal materials and aid in the quality control of HM. |
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Keywords: | Herbal medicine MarkerLynx MassFragment MSE Mycotoxin |
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