| 微渗析活体取样-NiHCF修饰电极色谱电化学检测的研究 |
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| 引用本文： | 施国跃,周红国,陆华,张季平,金利通.微渗析活体取样-NiHCF修饰电极色谱电化学检测的研究[J].分析化学,1998,26(7):814-818. |
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| 作者姓名： | 施国跃 周红国 陆华 张季平 金利通 |
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| 作者单位： | 华东师范大学化学与生命科学学院!上海200062 |
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| 基金项目： | 中国科学院长春应用化学研究所电分析化学开放研究实验室资助课题 |
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| 摘 要： | 研究了六氰合亚铁酸镍修饰电极对单胺类递质及其代谢产物的催化机理，首次以该 电极为色谱电化学检测器，对８种物质进行了分离测定。同时，将微渗析活体取样技术与高 效液相色谱一电化学检测联用测定了ＳＤ大鼠脑尾核处的单胺类递质及其代谢产物，并研究了 Ｃａ２＋浓度对脑中递质的影响，拓展了电分析化学在生物活体分析中的应用范围。
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| 关 键 词： | 六氰合亚铁酸镍 修饰电极 色谱 微渗析取样技术 大鼠脑尾核 单胺类递质 代谢产物 |
The Determination of Monoamine Transmitters and Their Metabolites In Vivo by Microdialysis Using High Performance Liquid Chromatography with Nickel Hexacyanoferrate Chemically Modified Electrode |
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| Shi Guoyue,Zhou Hongguo,Lu Hua,Zhang Jiping,Jin Litong.The Determination of Monoamine Transmitters and Their Metabolites In Vivo by Microdialysis Using High Performance Liquid Chromatography with Nickel Hexacyanoferrate Chemically Modified Electrode[J].Chinese Journal of Analytical Chemistry,1998,26(7):814-818. |
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| Authors: | Shi Guoyue Zhou Hongguo Lu Hua Zhang Jiping Jin Litong |
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| Abstract: | In this paper, we prepared a nickel hexacyanoferrate (NiHCF) chemically modified electrode (CME) and discussed its mechanism of the electrocatalytic oxidation to monoamine transmitters and their metabolites. The separation was performed on a C18 column with an eluting system containing phosphate buffer (pH = 5. 0) and methanol. A ed linear relationship was found between the concentration and peak heights of the monoamine transmitters and their metabolites from 10-8 mol/L to 10-5 mol/L. We coupled microdialysis sampling techique with LC and determined the monoamine transmitters and their metabolites of caudatum in freely moving rat. At the same time, we also studied the effect of Ca2+ on the transimmitters. |
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| Keywords: | Nickel hexacyanoferrate chemically modified elected chromatography microdialysis Sampling technique caudatum rat monoamine transmitters metabolites |
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