| 高分辨阿达玛变换显微荧光图像分析(Ⅱ)单细胞DNA荧光图像生成、处理及自动定量分析研究 |
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| 引用本文： | 周锦松,唐宏武,吴琼水,陈观铨.高分辨阿达玛变换显微荧光图像分析(Ⅱ)单细胞DNA荧光图像生成、处理及自动定量分析研究[J].分析科学学报,2003,19(1):17-20. |
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| 作者姓名： | 周锦松 唐宏武 吴琼水 陈观铨 |
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| 作者单位： | 武汉大学化学与分子科学学院测试中心,武汉,430072 |
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| 基金项目： | 国家自然科学基金 (No.2 0 0 75 0 1 9)，武汉市青年科技“晨光计划”项目 (995 0 0 4 0 78G) |
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| 摘 要： | 本研究把荧光显微镜，阿达玛变换多通道成像技术和计算机图象处理技术结合起来，建立了一种单细胞DNA荧光图像自动定量分析系统，对系统的成像原理、信号编码和解码方法及单细胞定量分析方法进行了讨论，分析结果表明，本系统提供的单细胞定量分析数据稳定可靠。
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| 关 键 词： | 阿达玛变换 荧光图像 DNA 显微图像 图像分析 定量分析 计算机图像处理 |
| 文章编号： | 1006-6144(2003)01-0017-04 |
| 修稿时间： | 2002年7月1日 |
High-resolution Hadamard Transform Microscopic Fluorescence Image Analysis( Ⅱ )Image Acquisition, Processing and Quantitative Analysis of DNA in Single Cells |
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| ZHOU Jin-song,TANG Hong-wu ,WU Qiong-shui,CHEN Guan-quan.High-resolution Hadamard Transform Microscopic Fluorescence Image Analysis( Ⅱ )Image Acquisition, Processing and Quantitative Analysis of DNA in Single Cells[J].Journal of Analytical Science,2003,19(1):17-20. |
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| Authors: | ZHOU Jin-song TANG Hong-wu WU Qiong-shui CHEN Guan-quan |
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| Institution: | ZHOU Jin-song,TANG Hong-wu *,WU Qiong-shui,CHEN Guan-quan |
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| Abstract: | In this study, by combining fluorescence microscope and image processing to Hadamard transform(HT) multiplexed imaging technique, an automatic system was established to quantitatively analyze DNA in single cells based on fluorescence imaging. The principle of Hadamard transform multiplexed imaging, the encoding and decoding of signal and the quantitative method of single cells were investigated in detail. It was found that the analytical results were reliable when the system was applied to cellular quantitative analysis. |
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| Keywords: | Hadamard transform Microscopic image Image analysis Quantitative analysis |
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