| Real-time Quantitative RT-PCR for CT9 Level in Human Cancer |
| |
| 作者姓名： | JIN Xiang-qun ZHANG Jing-min XU Hui ZHOU Yan WANG Guang-shu ZHAO Yan-qiu ZHANG Han-qi |
| |
| 作者单位： | JIN Xiang-qun 1，2，ZHANG Jing-min 2，XU Hui 2，ZHOU Yan 3，WANG Guang-shu 2，ZHAO Yan-qiu 4 and ZHANG Han-qi 1**1. College of Chemistry，Jilin University，Changchun 130012，P. R. China;2. College of Phamercy，Jilin University，Changchun 130021，P. R. China;3. College of Nurse，Jilin University，Changchun 130021，P. R. China;4. No. 2 Affiliated Hospital，Jilin University，Changchun 130021，P. R. China |
| |
| 基金项目： | Supported by the National Natural Science Foundation of China（ No. 30371757）. |
| |
| 摘 要： | Introduction Malignantdiseasesarecharacterizedbytheunreg ulatedgrowthoftransformedcells.Inrecentyears,dramaticinsightsintothemolecularmechanismsofthis phenomenonhavebeenachievedfrombasiccancerre search.Manycellularfunctionsareregulatedbychan gesingeneexpr…
|
| 关 键 词： | CT9 睾丸癌抗原 实时RT-PCR 治疗 癌细胞 子宫内膜腺癌 |
| 文章编号： | 1005-9040（2006）-02-185-04 |
| 收稿时间： | 2006-01-10 |
Real-time Quantitative RT-PCR for CT9 Level in Human Cancer |
| |
| JIN Xiang-qun ZHANG Jing-min XU Hui ZHOU Yan WANG Guang-shu ZHAO Yan-qiu ZHANG Han-qi.Real-time Quantitative RT-PCR for CT9 Level in Human Cancer[J].Chemical Research in Chinese University,2006,22(2):185-188. |
| |
| Authors: | Xiang-qun JIN Jing-min ZHANG Hui XU Yan ZHOU Guang-shu WANG Yan-qiu ZHAO Han-qi ZHANG |
| |
| Institution: | aCollege of Chemistry, Jilin University, Changchun 130012, P. R. China;bCollege of Phamercy, Jilin University, Changchun 130021, P. R. China;cCollege of Nurse, Jilin University, Changchun 130021, P. R. China;dNo. 2 Affiliated Hospital, Jilin University, Changchun 130021, P. R. China |
| |
| Abstract: | CT9 is a recently cloned cancer-testis antigen, which is a member of the bromodomain and extraterminal family. Each member of this protein family contains two N|terminal bromodomain motifs. We investigated the distribution of CT9 in different tissues and the possibility for it to be used as a potential therapeutic target in cancer treament. By using the real-time RT-PCR method and 18SrRNA as an internal standard, we analyzed the CT9 expression in several normal human tissues and in the tissues of patients suffering from cancer. The result of this study shows that the highest level of mRNA is only present in testis tissue because the CT9 expression has not been detected in other normal tissues. In 6 of 10 cases of gastric adenocarcinoma, in 3 of 10 cases of esophageal squamous cell carcinoma, in 2 of 9 cases of endometrial carcinoma and only in 1 of 12 cases of brain cancer, the low level expression of CT9 was detected. In none of the 12 cases of cervical squamous cell carcinoma, the expression of CT9 was detected. Since the high level expression of CT9 is only found in the normal testis tissue, but the low expression in cancer tissues, for example tissues of cervical squamous cell carcinoma, brain cancer, endometrial adenocarcinoma, esophageal squamous cell carcinoma, we conclude that CT9 cannot be used as a cancer therapeutic target molecule for cervical squamous cell carcinoma, brain cancer, endometrial adenocarcinoma, esophageal squamous cell carcinoma. |
| |
| Keywords: | CT9 Cancer-testis antigen Real-time RT-PCR |
| 本文献已被 CNKI 维普 万方数据 ScienceDirect 等数据库收录！ |
