三维荧光二阶校正方法用于体液和细胞培养基中五味子甲素含量的快速测定

采用三维激发发射荧光光谱结合自加权交替三线性分解(SWATLD)二阶校正方法, 对人体液样(血浆样及尿液样)和细胞培养基样中五味子甲素的含量进行了直接快速定量分析. 在血浆背景、尿液背景和细胞培养基背景共存下, 当分析体系的组分数分别选择2时, 用SWATLD二阶校正方法获得相应五味子甲素的平均回收率分别为(100.4±1.6)%, (100.5±6.3)%和(103.6±4.5)%. 实验结果表明, 此方法不仅能够较好地解决这些复杂分析体系因背景内源荧光性物质与待分析物光谱严重重叠所引起的难分辨的问题, 还可以用于直接快速准确定量分析.

Determination of Schizandrin A in Body Fluids and Cell Culture Media by Excitation-emission Matrix Fluorescence Coupled with Second-order Calibration

Schizandrin A, which has been shown to have anticarcinogenic activity and hepatoprotective effect is the active schisandra lignan from a traditional chinese medicine schisandra chinensis (wuweizi). A novel method for quantitative analysis of schizandrin A in human plasma samples, urine samples and cell culture media was proposed by combining excitation-emission matrix fluorescence spectra with second-order calibration method based on self-weighted alternating trilinear decomposition algorithm (SWATLD). Satisfactory results have been achieved, fully exploiting “second-order advantage”. The average recoveries of schizandrin A in human plasma samples, urine samples and cell culture media obtained by using SWATLD with N=2 are （100.4±1.6）%，（100.5±6.3）% and（103.6±4.5）%, respectively. It proved that SWATLD based second-order calibration methodologies can be feasible to directly quantify the content of schizandrin A in human plasma samples, urine samples and cell culture media. It could be applied to quantitative analysis of schizandrin A even in the presence of unknown interferences, without time-consuming and complicated pretreatment.