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| 中蜂囊状幼虫病毒结构蛋白预测与鉴定 | |
| 引用本文： | 马鸣潇,马臣,程健,谢振声,李明,费东亮.中蜂囊状幼虫病毒结构蛋白预测与鉴定[J].高等学校化学学报,2011,32(8):1768-1773. |
| 作者姓名： | 马鸣潇 马臣 程健 谢振声 李明 费东亮 |
| 作者单位： | 1. 辽宁医学院实验动物中心,锦州,121001 2. 赤峰市翁牛特旗中蒙医院,乌丹,024500 3. 中国科学院生物物理研究所蛋白质组学平台,北京I00101 |
| 基金项目： | 国家自然科学基金(批准号:30972200); 辽宁省教育厅项目(批准号:2009A464)资助 |
| 摘 要： | 采用反转录聚合酶链反应(RT-PCR)方法、生物信息学软件和质谱分析对中蜂囊状幼虫病毒(CSBV)辽宁株(CSBV-LN)全基时组进行了分子克隆、序列测定、分子生物学特性分析及结构蛋白预测和鉴定.结果表明,CSBV-LN全基因组序列长度为8863个核苷酸,编码一个大的开放阅读框,其中包括178 nt的5′非编码区的前导... |
| 关 键 词： | 中蜂囊状幼虫病毒 反转录聚合酶链反应 结构蛋白 质谱分析 |
| 收稿时间： | 2011-01-06 |
Prediction and Identification of Structural Proteins of Chinese Sacbrood Virus LN Isolate |
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| MA Ming-Xiao,MA Chen,CHENG Jian,XIE Zhen-Sheng,LI Ming,FEI Dong-Liang.Prediction and Identification of Structural Proteins of Chinese Sacbrood Virus LN Isolate[J].Chemical Research In Chinese Universities,2011,32(8):1768-1773. | |
| Authors: | MA Ming-Xiao MA Chen CHENG Jian XIE Zhen-Sheng LI Ming FEI Dong-Liang |
| Institution: | MA Ming-Xiao1,MA Chen2,CHENG Jian1,XIE Zhen-Sheng3,LI Ming1,FEI Dong-Liang1 (1.Department of Laboratory Animal Center Liaoning Medical University,Jinzhou 121001,China,2.Wengniuete Country Chinese Traditional Medicine and Mongolian Medicine in Chifeng Town,Wudan 024500,3.Proteomic Platform,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China) |
| Abstract: | Chinese sacbrood virus(CSBV) belongs to the small RNA virus family,the Picornaviridae.CSBV infection will cause lethal disease in individual bees or the collapse of entire colonies.But the CSBV was only finished sequence at the genetic level,and the structural proteins haven't been reported;thus it has restricted the prevention and control of CSBV.In the paper,the genome of CSBV purified from diseased insects was cloned and sequenced,and its molecular and biological characterization was analyzed,and its str... |
| Keywords: | Chinese sacbrood virus(CSBV) RT-PCR Structural protein Mass spectrographic analysis |
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