化学发光法测定奶粉中的三聚氰胺

在碱性条件下,三聚氰胺对鲁米诺-铁氰化钾体系化学发光反应具有明显的增敏作用;据此建立了流动注射化学发光测定奶粉中三聚氰胺的分析方法,确定了优化测定条件,并将该方法用于测定奶粉中的三聚氰胺.结果表明,在优化测定条件下,三聚氰胺的质量浓度在1.0×10-6～4.0×10-5 g.L-1范围内与发光强度呈现良好的线性关系,检出限为2.6×10-7 g.L-1,11次平行测定4.0×10-6 g.L-1三聚氰胺的相对标准偏差为3.42%.与此同时,将所建立的方法用于奶粉中三聚氰胺的测定,结果令人满意.

Determination of melamine in milk powder by chemiluminescence method

Melamine is able to significantly enhance the chemiluminescence reaction of luminal and K3Fe（CN）6 in alkaline medium.This makes it feasible to establish a new flow injection chemiluminescence method for determining melamine in milk powder.The optimal condition for the determination was examined,and as-established method was adopted to determine melamine in commercial milk powder.It was found that under the optimal experimental condition,melamine concentration in the range of 1.0×10-6-4.0×10-5 g · L-1 was linearly proportional to enhancing chemiluminescence signals.The detection limit for melamine was determined to be 2.6×10-7 g · L-1,and the relative standard deviation for 11 consecutive measurements of melamine with a concentration of 4.0×10-6 g · L-1 was 3.42%.In the meantime,as-established method was successfully applied to determine melamine in commercial milk powder.