Application of WET sequence for the detection of the ligand signals resonating close to water |
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| Authors: | Kazuo Furihata Sakurako Shimotakahara Yoichi Shibusawa Mitsuru Tashiro |
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| Institution: | 1. Division of Agriculture and Agricultural Life Sciences, The University of Tokyo, Yayoi, Bunkyo‐ku, Tokyo 113‐8657, Japan;2. School of Pharmacy, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo 192‐0392, Japan;3. Department of Chemistry, College of Science and Technology, Meisei University, Hino, Tokyo 191‐8506, Japan |
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| Abstract: | An efficient pulse sequence for observing the ligand signals resonating close to the water signal has been developed by incorporating the WET technique into the saturation transfer difference pulse sequence. Although several pulse sequences have been developed for observing a ligand binding with a protein receptor, the ligand signals resonating close to the water were undetectable owing to the interference of the huge water signal in the samples containing 95% 1H2O. On the point of sample preparation, it is preferable to avoid the solvent exchange in the protein samples. In the proposed pulse sequence, a WET sequence is incorporated for the selective suppression of the water resonance. The efficient water suppression and the clear observation of the bound ligand signals close to the water have been demonstrated using the lysozyme‐glucose complex. Copyright © 2009 John Wiley & Sons, Ltd. |
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| Keywords: | NMR 1H NMR NMR screening protein– ligand interactions WET sequence STD |
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