Purification and some properties of squalene-2,3-epoxide: lanosterol cyclase from rat liver |
| |
Authors: | M Kusano I Abe U Sankawa Y Ebizuka |
| |
Institution: | Faculty of Pharmaceutical Sciences, University of Tokyo, Japan. |
| |
Abstract: | Squalene-2,3-epoxide: lanosterol cyclase was purified from rat liver in five steps as a soluble and homogeneous protein. The purified enzyme showed a single band on SDS-polyacrylamide gel electrophoresis with a molecular weight of 75 kD. In its native state it behaved as a homo-dimer. The isoelectric point of 5.5 and the apparent Km value for (3S)-squalene-epoxide of 55 microM were estimated for the cyclase. |
| |
Keywords: | |
|
|