固定波长同步荧光光谱参数的理论计算

基于荧光激发光谱和发射光谱对波长呈高斯分布的设定,本文推导出固定波长同步荧光光谱峰峰值位置、相对强度和半峰宽度等3个主要光谱参数的理论计算式。所提出的计算式应用于若干荧光物质光谱参数的计算,并和实测值、文献计算值作了对照。结果表明,和文献计算方法相比,本法与所研究物质的实际光谱参数较为接近,可为固定波长同步荧光光谱参数的理论计算提供一有效方法。

Theoretical Calculation for the Spectral Parameters of Constant-wavelength Synchronous Fluorescence Spectra

On the assumption that the fluorescence excitation and emission spectra can be represented by Gaussian distributions versus wavelength. the theoretical equations were derived for the calculation of peak location, intensity and half-width in constant-wavelength synchronous fluorescence spectra. The proposed e-quations were applied to the calculation of the spectral parameters for some compounds and the calculated data were compared with the experimental data and the calculation based on the literature equations, which indicated that the proposed method could provide an effective alternative for the calculation of the spectral parameters in constant-wavelength synchronous fluorescence spectra.