在线扫集-胶束毛细管电动色谱法测定复方氨酚烷胺胶囊中的3种有效成分

以在线扫集-胶束毛细管电动色谱法(Sweeping-MEKC)测定了复方氨酚烷胺胶囊中的马来酸氯苯那敏、咖啡因和对乙酰氨基酚3种有效成分。考察了缓冲溶液pH值、SDS浓度、分离电压及进样时间等对分离效果的影响。优化条件:以未涂层熔融石英毛细管(55 cm×50μm,有效柱长35 cm)为分离柱;环境温度25℃;80 mmol/L十二烷基磺酸钠+20 mmol/L NaH2PO4(pH 2.2)+15%乙腈为缓冲体系,分离电压-20kV,进样时间60 s(H=20.0 cm),测量波长210 nm。在该条件下氯苯那敏、咖啡因和对乙酰氨基酚在25min内出峰,峰面积RSD均小于4%;线性范围分别为2.45～39.17、1.61～25.76、1.58～25.28 mg/L;检出限(S/N=3)分别达139、34、24μg/L,回收率分别为96%～101%、98%～102%、96%～102%。

Determination of Acetaminophen,Caffeine and Chlorphenamine Maleate in Compound Paracetamol and Amantadine Hydrochloride Capsules by Sweeping-MEKC Method

A sweeping-micellar electrokinetic chromatographic(sweeping-MEKC) method was used for the simultaneous separation and determination of chlorphenamine maleate,caffeine and acetaminophen in compound paracetamol and amantadine hydrochloride capsules.Effects of sodium dodecyl sulfate(SDS) concentration,separation voltage,injection time of sample,composition and concentration of background buffer on separation efficiency were optimized.An optimal separation of three compounds was obtained on an uncoated fused silica capillary column(50 cm×50 μm,effective length 35 cm) by using background buffer containing 80 mmol/L SDS,20 mmol/L NaH2PO4 and 15% acetonitrile at pH 2.2 with a constant temperature of 25 ℃.The detection wavelength was set at 210 nm,the separation voltage at-20 kV and the sample injection time for 60 s.The result indicated that the separation of three compounds was performed within 25 min with relative standard deviation(RSDs) of their peak areas less than 4%.The calibration curves showed good linearities in the ranges of 2.45-39.17 mg/L for chlorphenamine,1.61-25.76 mg/L for caffeine and 1.58-25.28 mg/L for acetaminophen,with detection limits(S/N=3) of 139,34,24 μg/L,respectively.The spiked recoveries for acetaminophen,caffeine and chlorphenamine maleate were 96%-101%,98%-102% and 96%-102%,respectively.