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Immobilized anthraquinone for redox mediation of horseradish peroxidase for hydrogen peroxide sensing
Authors:Yuehua Dou   Stephen Haswell   John Greenman  Jay Wadhawan  
Affiliation:aDepartment of Physical Sciences (CHEMISTRY), The University of Hull, Cottingham Road, Kingston-upon-Hull HU6 7RX, United Kingdom;bCentre for Biomedical Research, Postgraduate Medical Institute, The University of Hull, Cottingham Road, Kingston-upon-Hull HU6 7RX, United Kingdom
Abstract:The detection of hydrogen peroxide is detailed using horseradish peroxidase and anthraquinone. Both species are immobilized on a glassy carbon electrode substrate. This dual immobilization gives rise to lower detection limits compared with the situation when either of the species is immobilized. Detection limits of 40 nM are reported within physiologically-relevant media.
Keywords:Hydrogen peroxide   Biosensor   Anthraquinone   Horseradish peroxidase
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