黄花倒水莲皂甙Reinioside C在大鼠体内代谢产物的鉴定

为了探讨五环三萜皂甙ReiniosideC在大鼠体内代谢产物的结构,单剂量口服ReiniosideC,5h后放血处死,用制备型高效液相色谱方法对血液和各脏器进行提取分离,从大肠内容物和粪中,分离得到了7种代谢产物.用FAB-MS,¹H NMR和¹³C NMR方法鉴定了它们的结构.

Identification of Metabolites of Reinioside C, A Triterpene Saponin from the Roots of Polygala fallax Hemsl

Triterpene saponins were widely distributed in the oriental traditional crude drugs, which play important roles as anti-inflammatory, anticancer and antihepatitis agent. For example, from Ginseng Radix, Bupleuri Radix, Polygalae Radix, Platycodi Radix etc, many bioactive and available triterpene saponins were obtained and they could explain the effect of the crude drug. However metabolic transformation of triterpene saponin in vivo have not been reported. We studied the metabolism of reinioside C, a triterpene saponin from the roots of Polygala fallax Hemsl in rats. After oral administration of reinioside C, seven metabolites(RA-RG) of reinioside Cwere obtained from the content of large intestin and feces. The structures of metabolites were elucidated as 28-O-α Lrhamnopyranosyl-(1→2)-(3,4 diacetyl)-β-Dfucopyranosyl presenegin, 28-O-β-Dxylopyranosyl-(1→4)-α-Lrhamnopyranosyl-(1→2)-(3,4 diacetyl)-β-Dfucopyranosyl presenegin, 28-O-α-Lrhamnopyranosyl-(1→2) (3,4-diacetyl)-β-Dfucopyranosyl presenegin₃ O-β-Dglucopyranosyl-(1→2)-β-Dglucopy ranoside, 28-O-β-Dxylopyranosyl-(1→4)-α-Lrhamnopyranosyl-(1→2)-β-D-fucopyranosyl presenegin 3-O-β-Dglucopyranosyl-(1→2)-β-Dglucopyranoside, 28-O-β-Dxylopyranosyl-(1→4)-α-Lrhamnopy ranosyl-(1→2)-(3 acetyl)-β-D-fucopyranosyl presenegin-3-O-β-D-glu copyranosyl-(1→2)-β-D-glucopyranoside, 28-O-β-D-xylopyranosyl-(1→4)-α-Lrhamnopyranosyl-(1→2)-(4 acetyl)-β-D-fucopyranosyl pre senegin₃ O-β-D-glucopyranosyl-(1→2)-β-Dglucopyranoside, 28-O-β-D-fucopyranosyl presenegin, respectively, on the basis of spectroscopic data.