Separation of Proteins and Other Compounds by Capillary Isotachophoresis

Electrophoresis, as the name implies, consists essentially in moving charged particles under the influence of an electric field. Since the first practical experiments by Tiselius¹, most of the variations in electrophoretic techniques have been based on the principle of zone electrophoresis. Only in the last decade did electrophoretic separation techniques, based on other principles, become available; isoelectric focusing and isotachophoresis. It is becoming more and more clear that neither of these techniques can provide the ideal solution to every protein separation problem but that each of them has its special field of application where it can give the researcher a maximum of information.