Determination of bromadiolone in pheasants and foxes by differential pulse voltammetry |
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| Authors: | Miroslava Beklova Sona Krizkova Veronika Supalkova Radka Mikelova Vojtech Adam Jiri Pikula |
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| Affiliation: | 1. Faculty of Veterinary Hygiene and Ecology, Department of Veterinary Ecology and Environmental Protection , University of Veterinary and Pharmaceutical Sciences , Palackeho 1–3, CZ-612 42, Brno, Czech Republic;2. Faculty of Agronomy, Department of Chemistry and Biochemistry , Mendel University of Agriculture and Forestry , Zemedelska 1, CZ-613 00, Brno, Czech Republic;3. Faculty of Agronomy, Department of Chemistry and Biochemistry , Mendel University of Agriculture and Forestry , Zemedelska 1, CZ-613 00, Brno, Czech Republic;4. Faculty of Agronomy, Department of Plant Biology , Mendel University of Agriculture and Forestry , Zemedelska 1, CZ-613 00, Brno, Czech Republic;5. Faculty of Science, Department of Theoretical and Physical Chemistry , Masaryk University , Kotlarska 2, CZ-611 37, Brno, Czech Republic;6. Faculty of Veterinary Hygiene and Ecology, Department of Veterinary Ecology and Environmental Protection , University of Veterinary and Pharmaceutical Sciences , Palackeho 1–3, CZ-612 42, Brno, Czech Republic;7. Faculty of Science, Department of Genetics and Molecular Biology , Masaryk University , Kotlarska 2, CZ-611 37, Brno, Czech Republic |
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| Abstract: | Bromadiolone, a commercially used anticoagulant rodenticide, was determined in tissues of various animals by differential pulse voltammetry with a carbon-paste electrode. Under the most suitable experimental conditions (step potential of 25?mV?s?1 and 0.2?mol?L?1 acetate buffer, pH 4.2), the limit of detection was 0.5?ng?mL?1. The electroanalytical method was consequently used to investigate the bromadiolone transport within the food chain. Pheasants were exposed to bromadiolone and then used as feed for a fox. The average levels were 528 and 198?ng of bromadiolone per gram of fresh weight of liver of pheasant and fox, respectively. Due to the surprisingly lower content of bromadiolone in the latter, a basic biochemical analysis, particularly blood coagulation, was performed. Among the parameters studied (thrombin time, prothrombin time, activated partial thromboplastin time, and fibrinogen), there was prolongation of the prothrombin time and activated partial thromboplastin time. |
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| Keywords: | Bromadiolone Superwarfarines Differential pulse voltammetry Cyclic voltammetry Carbon-paste electrode Electrochemistry Animals |
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