EFFECT OF FAR-UV AND NEAR-UV RADIATION ON THE CELL SURFACE CHARGE OF THE PROTOZOAN |
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Authors: | Fernando Costa E Silva Filho Cezar Antonio Elias Wanderley de Souza † |
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Institution: | Instituto de Biofísica, Universidade Federal do Rio de Janeiro, Centro de Ciências de Saúde—Bloco G, Ilha do Fundo, CEP. 21941, Rio de Janeiro. Brasil |
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Abstract: | Abstract— Cell electrophoresis was used to detect the effect of far-UV or near-UV radiation on the cell surface charge of the pathogenic protozoan Tritrichomonas foerus . Either far-UV or near-UV radiation interfered with the surface charge of T. foetus at fluences which inhibited cell growth by 50%. Both UV-radiations induced a significant decrease on surface charge of T. foetus , as evaluated by measurement of its electrophoretic mobility (EPM). Determinations of EPM of protozoa in solution of low ionic strength indicated that the decrease in the EPM induced by far-UV is much less pronounced than that observed for near-UV or control cells. |
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