面向DNA甲基化修饰分析的质谱技术

DNA甲基化作为表观遗传修饰中一种重要的调控方式，通过调控基因的表达，从而影响机体内一系列的生物学过程。色谱-质谱法是研究DNA甲基化修饰的重要研究手段。随着对哺乳动物DNA甲基化的生物学功能的深入研究，应用于研究表观遗传修饰的手段与仪器设备越来越先进。为了对DNA修饰进行定性与定量的分析检测，除了高效液相色谱整合不同种类质量分析器的质谱联用（HPLC-MS）技术外，目前还开发应用了基质辅助激光解析质谱技术（MALDI-ToF-MS）和气相色谱-质谱联用技术（GC-MS），从而极大拓展了DNA甲基化修饰研究的手段。本文对分析表观遗传DNA甲基化修饰的质谱技术发展进行综述，希望为DNA甲基化修饰分析提供有价值的研究策略。

StudyonDNAMethylationModificationAnalysisBasedonMassSpectrometry

As an important regulation mode in epigenetic modification, DNA methylation affects a series of biological processes in living systems by regulating gene expression. The chromatography tandem -mass spectrometry is an important technology to study DNA methylation modification. With delving into the biological functions of mammalian DNA methylation, more and more advanced approaches and instruments are used to study epigenetic modification. In order to carry out qualitative and quantitative analysis and detection of DNA modification, in addition to high performance liquid chromatography tandem mass spectrometry technology (HPLC-MS) integrating different kinds of quality analyzers, matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-ToF-MS) and gas chromatography tandem mass spectrometry (GC-MS) have also been developed and applied in this area, thus greatly expanding the methods for DNA methylation modification investigations. This paper summarizes the advancements of mass spectrometry technology for studying epigenetic DNA methylation modification, hoping to provide valuable research strategies for DNA methylation modification.