| 中国普通野生稻线粒体DNA的限制性片段长度多态性 |
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| 引用本文： | 朱世华,汪向明,王明全.中国普通野生稻线粒体DNA的限制性片段长度多态性[J].宁波大学学报(理工版),1998(4). |
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| 作者姓名： | 朱世华 汪向明 王明全 |
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| 作者单位： | 宁波大学生物系(朱世华)，武汉大学生命科学学院遗传学系(汪向明，王明全) |
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| 基金项目： | 华中农业大学作物遗传改良国家重点实验室部分经费资助 |
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| 摘 要： | 用BamHI／H454、BamHI／pQT2-7-1、EcoRI／B376和EcoRI／pQT12四种酶／探针组合对85份普通野生稻、亚洲栽培稻和橹稻进行了线粒体DNA的限制性片段长度多态性的分析．共检测到16条多态性带，15种表型，组合成14种线粒体DNA变异类型．其中抽稻和粳稻的线粒体DNA已分化，培稻偏粳型，大部分普通野生稻属（或偏）拉型，但江西的东乡普通野生稻类型比较独特．中国普遍野生稻的线粒体DNA与地理分布相关，湖南的江永、茶陵和江西的东乡等不同的群体以地理位置相聚，随地理梯度遗传分化由少到多．
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| 关 键 词： | 普通野生稻 线粒体DNA 限制性片段长度多态性 遗传多样性 |
MITOCHONDRIAL DNA RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN Oryza rufipogon OF CHINA |
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| Zhu Shihua.MITOCHONDRIAL DNA RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN Oryza rufipogon OF CHINA[J].Journal of Ningbo University(Natural Science and Engineering Edition),1998(4). |
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| Authors: | Zhu Shihua |
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| Abstract: | Eighty-fivc accessions of O. rufipogon, O. sativa and Ludao are assayed f0r restrichon fYagment length polymorphism (RFLP) of the mitochondrial DNA(mtDNA)for Bam Hl/H454, Bcm HI/pQT2-7-1, EcoRI/B376 and ECoRI/pQTl2. sixtCen polymorphic bands and fiftccn phenotypes arc detected, which consist of 14 types of mitochondrial DNAs variation. There are differenccs in mit0chondrial DNA betWeen jrporyica and indica, and the type of Ludao is similar to joponica rice. The types of mitochondrial DNA variation seem to correspond wcll to the geograPhical distribuhons,while the comparative analysis of 0. ru/40gon populations in Jiangyong, Chaling of Hunan and Dongxiang of Jiangxi show the incleasing tcndency of genetic differenhation. |
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| Keywords: | 0ryza rufipogon mitochondrial DNArestriction fragment length polymorphism (RFLP) genetic diversity |
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