Optimization of stepwise gradient elution in reversed-phase chromatography |
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Authors: | W Go?kiewicz |
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Institution: | (1) Department of Inorganic and Analytical Chemistry, Medical Academy, 20-081 Lublin, Poland |
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Abstract: | Summary Equations describing multi-step gradient elution with a mobile phase of constant composition in each step were derived. These
equations useful for calculating the retention volumes in both gradient HPLC and TLC were derived on the basis of the relationship
between the isocratic capacity factor and the volume fraction of the organic modifier. The validity of the equations was experimentally
verified in a LiChrosorbRP-18-water/methanol system for 11 methyl- and chlorobenzenes and phenols. A satisfactory agreement
between the theoretical and experimental k′ values was found. |
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Keywords: | Reversed-phase chromatography Column liquid chromatography Isocratic elution Optimization of stepwise elution Chloro- and methylphenols |
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