Influence of Extruder Temperature and Screw Speed on Pretreatment of Corn Stover while Varying Enzymes and Their Ratios |
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Authors: | Chinnadurai Karunanithy and Kasiviswanathan Muthukumarappan |
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Institution: | (1) Agricultural & Biosystems Engineering Department, South Dakota State University, Brookings, SD 57007, USA |
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Abstract: | Pretreatment is being the first and most expensive step, it has pervasive impacts on all other steps in overall conversion
process. There are several pretreatment methods using physical, chemical, and biological principles which are under various
stages of investigation. Extrusion can be used as one of the physical pretreatment methods towards biofuel production. The
objective of this study was to evaluate the effect of barrel temperature and screw speed on sugar recovery from corn stover,
to select a suitable enzyme combination and its ratio. Corn stover was pretreated in a single screw extruder with five screw
speeds (25, 50, 75, 100, and 125 rpm) and five barrel temperatures (25, 50, 75, 100, and 125 °C). In order to select a suitable
enzyme combination and ratio, different levels of cellulase and β-glucosidase, multienzyme complex and β-glucosidase were
used during saccharification of pretreated corn stover. From the statistical analysis, it was found that screw speed and temperature
had a significant effect on sugar recovery from corn stover. Higher glucose, xylose, and combined sugar recovery of 75, 49,
and 61%, respectively, were recorded at 75 rpm and 125 °C. This pretreatment condition resulted in 2.0, 1.7, and 2.0 times
higher than the control sample using 1:4 cellulase and β-glucosidase combination. |
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