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| Cloned s-Lap Gene Coding Area, Expression and Localization of s-Lap／GFP Fusion Protein in Mammal Cells | |
| 作者姓名： | SONGYi-shu SONGZhi-yu LIHong-jun WuYin BAOYong-li TANDa-peng LIYu-xin |
| 作者单位： | [1]InstituteofGeneticsandCytology,NortheastNormalUniversity,Changchun130024,P.R.China [2]DepartmentofUrology,theFirstHospital,JilinUniversity,Changchun130021,P.R.China [3]China-JapanUnionHospital,JilinUniversity,Changchun130023,P.R.China |
| 基金项目： | 中国博士后科学基金，吉林省自然科学基金 |
| 摘 要： | s-Lap is a new gene sequence from pig retinal pigment epithelial (RPE) cells, which was found and cloned in the early period of apoptosis of RPE cells damaged with visible light. We cloned the coding area sequence of the novel gene of s-Lap and constructed its recombinant eukaryotic plasmid pcDNA3.1-GFP/s-lap with the recombinant DNA technique. The expression and localization of s-lap/GFP fusion protein in CHO and B16 cell lines were studied with the instantaneously transfected pcDNA3.1-GFP/s-lap recombinant plasmid. s-Lap/GFP fusion protein can be expressed in CHO and B16 cells with a high rate expression in the nuclei. |
| 关 键 词： | S-LAP基因 编码 蛋白质 细胞 哺乳动物 色素 |
| 收稿时间： | 2004-10-28 |
Cloned s-Lap Gene Coding Area, Expression and Localizationof s-Lap/GFP Fusion Protein in Mammal Cells |
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| SONGYi-shu SONGZhi-yu LIHong-jun WuYin BAOYong-li TANDa-peng LIYu-xin.Cloned s-Lap Gene Coding Area, Expression and Localizationof s-Lap/GFP Fusion Protein in Mammal Cells[J].Chemical Research in Chinese University,2005,21(3):298-300. | |
| Authors: | SONG Yi-shu SONG Zhi-yu LI Hong-jun Wu Yin BAO Yong-li TAN Da-peng LI Yu-xin |
| Institution: | 1. Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, P. R. China; 2. Department of Urology, the First Hospital, Jilin University, Changchun 130021, P. R. China; 3. China-Japan Union Hospital, Jilin University, Changchun 130023, P. R. China |
| Abstract: | s-Lap is a new gene sequence from pig retinal pigment epithelial(RPE) cells, which was found and cloned in the early period of apoptosis of RPE cells damaged with visible light. We cloned the coding area sequence of the novel gene of s-Lap and constructed its recombinant eukaryotic plasmid pcDNA3.1-GFP/s-lap with the recombinant DNA technique. The expression and localization of s-lap/GFP fusion protein in CHO and B16 cell lines were studied with the instantaneously transfected pcDNA3.1-GFP/s-lap recombinant plasmid. s-Lap/GFP fusion protein can be expressed in CHO and B16 cells with a high rate expression in the nuclei. |
| Keywords: | s-Lap gene Fusion protein Mammal cell Expression Localization |
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