Identification of aminophospholipid stereomers by positive-ion fast atom bombardment combined with collisional activation mass-analysed ion kinetic energy analysis and high-performance liquid chromatography

Two methods for the identification of aminophospholipid stereomers are described. After a chemical derivatization, 9-fluorenylmethoxycarbonyl derivatives of diacyl-sn-2- and diacyl-sn-3-phosphoserine and diacyl-sn-2- and diacyl-sn-3-phosphoethanolamine can be characterized by positive-ion fast atom bombardment combined with collisional activation mass-analysed ion kinetic energy analysis based on the differentiation of relative abundances [M + H + diethanolamine ? 89]⁺ and [M + H]⁺ fragments derived from [M + H + diethanolamine]⁺ ions, the protonated solvated molecules, and normal phase high-performance liquid chromatography on the basis of different elution times of the derivatives of the aminophospholipid stereomers on an aminopropyl-bonded column.