Determination of polyamines by high-performance liquid chromatography with chemiluminescence detection |
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| Authors: | Qiuhua Wu Yuqin Su Lihua Yang Jingci Li Jingjun Ma Chun Wang Zhengping Li |
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| Affiliation: | (1) College of Science, Agricultural University of Hebei, 071001 Baoding, Hebei, P.R. China;(2) College of Chemistry and Environmental Science, University of Hebei, 071002 Baoding, Hebei, P.R. China |
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| Abstract: | A method was developed for the determination of polyamines (PA) by high-performance liquid chromatography with chemiluminescence detection. It is based on the unsaturated complex of PA with Cu(II) which had a strong catalytic effect on the luminol-H2O2 chemiluminescence reaction. The separation of PA was carried out on a reveres phase C18 column using methanol/water (25/75, v/v) as a mobile phase. The method was applied to the analysis of putrescine and the total amount of spermine and spermidine in apple leaves and strawberry fruit. The results indicated that the method is practical and useful. |
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| Keywords: | : Chemiluminescence detection HPLC polyamines Cu(II) |
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