A Validated RP–LC Method for Simultaneous Determination of Losartan Potassium and Amlodipine Besilate in Pharmaceutical Preparations |
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Authors: | Carlos F Pedroso Julcemara G de Oliveira Francinete R Campos Alan G Gon?alves Angela C L B Trindade and Roberto Pontarolo |
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Institution: | (1) Departamento de Farm?cia, Universidade Federal do Paran?, Curitiba, Paran?, Brazil |
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Abstract: | A simple RP–LC method for simultaneous quantification of losartan and amlodipine and separation of their degradation products
has been developed. For this purpose we tested appropriated mobile phase pH range, flow rate, temperature and different columns.
The method was validated with an ODS column. A gradient of acetonitrile and phosphate pH 3.0 buffer was utilized as mobile
phase. The linearity was determined at 50–150% level. Individual recoveries at 70–130% level ranged from 98.8 to 100.5% for
losartan and 96.4–101.2% for amlodipine. The robustness was also evaluated. Although losartan has much higher quantities than
amlodipine in commercial tablets, this method allowed simultaneous quantification for both drugs. |
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