Micro-Electrospray: Zeptomole/attomole per microliter sensitivity for peptides |
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| Authors: | Per E Andren Mark R Emmett Richard M Caprioli |
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| Institution: | 1. Department of Biochemistry and Molecular Biology, University of Texas Medical School, P.O. Box 20708, 77225, Houston, TX
2. Analytical Chemistry Center, University of Texas Medical School, 6431 Fannin Street, Houston, Texas, USA
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| Abstract: | The micro-electrospray ionization source has been optimized for the specific analysis of neuropeptides such as neurotensin and methionine enkephalin. The source has the option of integrating nanoliter flow-rate desalting and preconcentration techniques into the micro-electrospray spray needle, eliminating post-column dead volumes. For neurotensin, the most sensitive neuropeptide analyzed thus far in this work, the injection of 10 μL of a solution containing 320 zeptomolesy/gmL gave an M + 3H]+3 ion at m/z 558.4 with S/N of > 8∶1. The MS/MS analysis of this peptide for the fragment ion at m/z 578.9 gave a S/N > 20∶1 for a solution containing 32 attomoles/μL. |
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