Simultaneous Determination of Aldoses and Uronic Acids of Citrus Pectin by LC with Precolumn Derivatization and UV Detection |
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| Authors: | Jianjun Li Jing Sun Zhongfu Wang Linjuan Huang |
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| Institution: | 1. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Life Science College, Northwest University, 710069, Xi’an, China
2. Department of Chemistry, College of Science, Xi’an Jiaotong University, 710049, Xi’an, China
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| Abstract: | The simultaneous determination of aldoses and uronic acids is now available by liquid chromatography. The procedure involves acid hydrolysis followed by derivatization with 3-amino-9-ethylcarbazole, which was first employed for aldoses and uronic acids derivatization. The usefulness of this method is seen in the ability to analyze commercially available citrus pectin. The results show that citrus pectin consists of xylose, glucose, arabinose, rhamnose, galactose and galacturonic acid in molar ratios of 0.3:1.0:1.8:2.8:7.4:50.9, which was consistent with the result obtained by GC. The described method is suitable for routine analysis of pectin or other polysaccharides containing uronic acids. |
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