Preparation of β-cyclodextrin sulfate-immobilized hydrophilic vinyl-polymer gel as a selective,high recovery and stable adsorbent for high-performance affinity chromatography of heparin-binding substances |
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| Authors: | Ishimura K Fukunaga K Ohta T Nakamura H Irie T Uekama K |
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| Institution: | (1) Department of Drug Formulations, Kaken Pharmaceutical Co. Ltd., 301 Gensuke, Fujieda-Shi, 426 Shizuoka, Japan;(2) Faculty of Pharmaceutical Sciences, Science University of Tokyo, 12 Ichigaya-Funagawara-Machi, Shinjuku-ku, 162 Tokyo, Japan;(3) Faculty of Pharmaceutical Sciences, Kumamoto University, 5-1 Oe-honmachi, 862 Kumamoto, Japan |
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| Abstract: | Summary β-Cyclodextrin sulfate having heparin-mimicking activity was immobilized on a hydrophilic vinyl-polymer gel, TSKgel AF-Epoxy,
TOYOPEARL 650M. A column packed with this material could be used for high-performance affinity chromatographic separation
of heparin-binding substances such as growth factors, enzymes, coagulation factors and lipoproteins. The substances retained
on the column were recovered more easily than those on a heparin-immobilized column. Furthermore, the β-Cyclodextin sulfate-immobilized
gel was superior in stability to the heparin-immobilized gel when exposed to acidic and basic solutions. |
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| Keywords: | Affinity chromatography β -Cyclodextrin sulfate Heparin-binding substances Fibroblast growth factor |
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