Separation of urea and other polar compounds by HPLC |
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| Authors: | M Pálfi-Ledniczky G Dénes K Ujszászy |
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| Institution: | (1) Central Research Institute for Chemistry of the Hungarian Academy of Sciences, P. O. Box 17, H-1525 Budapest, Hungary;(2) Spectroscopic Department, EGIS Pharmaceutical Factory, P. O. Box 100, H-1475 Budapest, Hungary |
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| Abstract: | Summary Urea, glycolic and aminoxyacetic acid amides are the polar metabolites of 2-acetyl-3-phenyl-tetrahydro-1,2,4-oxadiazin-5-one
(RGH 4615). They cannot be separated on octadecyl-, cyanosilyl- or amino-phase columns, but silica, used with C3-C4 alcohol and water mixtures as the eluent permits their separation. Besides refractive index detection and on-line radioactivity
measurement the metabolic formation of14C-urea was proved by enzymatic cleavage into14CO2. |
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| Keywords: | Column liquid chromatography Urea Silica phase |
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