| 甲基莲心碱与人血清白蛋白相互作用的研究 |
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| 引用本文： | 周能,梁逸曾,刘韶. 甲基莲心碱与人血清白蛋白相互作用的研究[J]. 光谱实验室, 2010, 27(1): 49-54 |
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| 作者姓名： | 周能 梁逸曾 刘韶 |
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| 作者单位： | 玉林师范学院化学与生物系天然产物研究所,广西玉林市教育中路299号,537000;中南大学化学化工学院中草药现代化研究中心,长沙市,410083;中南大学化学化工学院中草药现代化研究中心,长沙市,410083;中南大学湘雅医院药剂科,长沙市,410008 |
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| 基金项目： | 国家自然科学基金重点项目(No20235020);;教育部重点科技项目培育基金(No.704036);;广西教育厅科研项目(No.200507217) |
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| 摘 要： | 荧光光谱、圆二色谱等多种光谱技术研究甲基莲心碱(NF)与人血清白蛋白(HSA)的相互作用,以Stern-Volmer和Lineweaver-Burk方程处理数据。甲基莲心碱对人血清白蛋白有较强的荧光猝灭作用并为静态猝灭,主要作用力为疏水作用和静电作用。309K下NF与HSA相互作用的结合常数为1.39×104L.mol-1,NF-色氨酸残基之间的距离为2.70nm,热力学参数ΔH0=-13.0KJ·mol-1,ΔS0=37.2J.(K.mol)-1。NF的结合使蛋白α-螺旋百分数增加。中药活性成分甘草次酸等和内源脂肪酸对结合的影响较少。
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| 关 键 词： | 甲基莲心碱 人血清白蛋白 光谱学方法 甘草次酸 |
Study on the Interaction of Neferine with Human Serum Albumin |
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| ZHOU Neng,LIANG Yi-Zeng,LIU Shao. Study on the Interaction of Neferine with Human Serum Albumin[J]. Chinese Journal of Spectroscopy Laboratory, 2010, 27(1): 49-54 |
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| Authors: | ZHOU Neng LIANG Yi-Zeng LIU Shao |
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| Affiliation: | Institute of Natural Products;Department of Chemistry-Biology;Yulin Normal University;Yulin;Guangxi 537000;P.R.China;Research Center of Modernization of Chinese Medicines;College of Chemistry and Chemical Engineering;Central South University;Changsha 410083;P.R.China;Department of Pharmacy of Xiangya Hospital;Changsha 410008;P.R.China |
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| Abstract: | With the help of multitude spectroscopic techniques,such as fluorescence spectra and circular dichroism,the interaction between neferinr(NF)and human serum albumin(HAS)was studied.Experimental datas were dealed with Stern-Volmer plot and Line weaver-Burk equation.The NF has a powerful ability to quench the albumin's fluorescence in a static mode and the main binding forces were hydrophobic interaction and electrostatic action.Under 309K,the binding constant was 1.39×10~4 L·moL~(-1).Distance between NF and tryptophan residue was 2.70nm.Thermodynamic parameters were AH0=-13.0kJ·moL~(-1),△S~0=37.2J·(K·mol)~(-1).Binding of NF with HAS can increase the percentage of α-helix of HAS.There was little effect of bioactive components in traditional Chinese medicine(glycyrrhetinic acid etc)and fatty acids on the binding. |
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| Keywords: | Neferine Human Serum Albumin Spectroscopic Method Glycyrrhetinic Acid |
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